Biology · Chapter 2: The Scientific Method (Solving a Biological Problem)

Steps of the Scientific Method

The scientific method step by step: observation, question, hypothesis, deduction, experiment, results and conclusion, with an example and a flow chart.

Taught in Class 9Class 11

Key termRememberExampleWatch out

01Concept

Biologists do not guess answers. They follow a careful, step-by-step way of solving problems called the scientific method. It is an organised way of finding answers to questions about nature by observing, testing and checking.

The same steps are used to find the cause of a disease, test a new medicine or find out why the leaves of a plant are turning yellow.

The steps of the scientific method. If the results do not support the hypothesis, the scientist goes back and makes a new one.

The steps

StepWhat the biologist doesExample: yellow leaves on a lemon plant
1. ObservationNotices something using the senses or instrumentsThe leaves of a lemon plant in the school garden are turning yellow.
2. QuestionAsks a clear question about itWhy are the leaves turning yellow?
3. HypothesisSuggests a possible answer that can be testedThe plant is not getting enough nitrogen from the soil.
4. DeductionWorks out what should happen if the hypothesis is true: "If…, then…"If the plant lacks nitrogen, then adding a nitrogen fertiliser will make new leaves green.
5. ExperimentTests the deduction, using a control groupGive fertiliser to some plants and none to others; keep everything else the same.
6. ResultsRecords what happens, as tables and graphsNew leaves on fertilised plants are green; the others stay yellow.
7. ConclusionDecides whether the hypothesis is accepted or rejectedThe hypothesis is accepted: the plants lacked nitrogen.

Sharing the results

At the end, scientists publish their work in scientific journals so that other scientists can repeat the experiment and check it. A result that others cannot repeat is not accepted.

02Virtual Lab

Try this topic for yourself in the virtual lab. It opens on its own page with steps, tasks and questions.

03Real World Examples

Testing a new wheat variety

Before a new wheat seed is given to farmers in Punjab and Sindh, research institutes such as the National Agricultural Research Centre (NARC) in Islamabad grow it beside ordinary wheat for several seasons and compare the yield. That is the scientific method at work.

Medicines

Every medicine sold in a pharmacy was first tested on volunteers: one group got the medicine and a control group got a dummy tablet (placebo). Only if the medicine worked better was it approved.

Dengue in Lahore and Karachi

Health workers observed that dengue cases rise after the monsoon. Testing the idea that standing water breeds the Aedes mosquito led to campaigns to empty water from tyres, pots and coolers.

04Practice Questions

Try to answer in your own words first, then open the model answer.

Short questions

Q1.Define the scientific method.Show answer

Answer

The scientific method is an organised, step-by-step way of finding answers to questions about nature by observation, hypothesis and experiment.

Q2.List the steps of the scientific method in the correct order.Show answer

Answer

  1. Observation
  2. Question (recognising the problem)
  3. Hypothesis
  4. Deduction
  5. Experiment
  6. Results (data)
  7. Conclusion
Q3.What happens if the results of an experiment do not support the hypothesis?Show answer

Answer

The hypothesis is rejected. The scientist makes a new hypothesis and tests it with new experiments.

Q4.Why do scientists publish their results?Show answer

Answer

So that other scientists can repeat the experiment and check the results. A result is accepted only when others get the same answer.

Q5.A student sees that the bread in her kitchen grows mould faster in the monsoon. Write a hypothesis and a deduction for this observation.Show answer

Answer

  • Hypothesis: mould grows faster when the air is humid (moist).
  • Deduction: if mould grows faster in humid air, then bread kept in a damp box will grow mould sooner than bread kept in a dry box.

Long questions

Q6.Describe the steps of the scientific method with the help of an example.Show answer

Answer

Example: the leaves of a lemon plant are turning yellow.

  1. Observation: the leaves are turning yellow.
  2. Question: why are the leaves turning yellow?
  3. Hypothesis: the plant is short of nitrogen.
  4. Deduction: if it lacks nitrogen, then nitrogen fertiliser will make new leaves green.
  5. Experiment: some plants get fertiliser (experimental group), others get none (control group). Water, light and soil are kept the same.
  6. Results: new leaves on fertilised plants become green; the others stay yellow.
  7. Conclusion: the hypothesis is accepted. If the results had been different, a new hypothesis would be made and tested.

05Quiz

Question 1 of 7
The first step of the scientific method is: